Dropdownlist doesn't fire the onselectedIndexChange

Hi,
I'm having a problem with two dropdownlist, one dependent of another.
I have this on my aspx file

<%@ Page Language="C#" MasterPageFile="~/MasterPage.master" AutoEventWireup="true" CodeFile="MantenimientoUsuarios.aspx.cs" Inherits="MantenimientoUsuarios" EnableViewState="true" %> <%@ Register Assembly="AjaxControlToolkit" Namespace="AjaxControlToolkit" TagPrefix="asp" %> <asp:Content ID="CtnPlaHoldPpal" ContentPlaceHolderID="CtnPlaHoldPpal" Runat="Server"> <asp:toolkitscriptmanager ID="ToolkitScriptManager1" runat="server" EnablePartialRendering="true"></asp:toolkitscriptmanager> <div class="breadcrumbs& ...