Where should I called DataBind or Page.DataBind() in my C# code behind

I have a lot of statements that set properties of certain controls throught my aspx page. I have figured out now that since I do not have a Grid on my page that I need to call DataBind() or Page.DataBind(); at some point.  Where is the best place to do this? Can it mess up anything else down the road?
TemporaryFolder='<%# UIHelper.GetTelerikAsyncTempUploadDirectory("fix...") %>' If I don't call Page.DataBind() or DataBind() then the TempFolder property will not be set for my RadAsyncUpload control.