4.5 Framework Error 404 - File or directory not found. The resource you are looking for might have been removed, had its name changed, or is temporarily unavailable.

Hello ,
i have basic web application in 4.5 asp.net in my local machine work fine after uploaded in my windows server 2008 R2 getting error 4.5 Framework Error 404 - File or directory not found. The resource you are looking for might have been removed, had its name changed, or is temporarily unavailable. others 4.0 application work fine .
for remove this error m update framework 4.5 but can't resolve this error.
Please send me suggestions about this error
Thanks in advance