<asp:DropDownList ID="DropDownList1" runat="server">
</asp:DropDownList>
SqlConnection objcon = new SqlConnection(".........");
objcon.Open();
SqlCommand objcmd = new SqlCommand("select * from Tablename", objcon);
SqlDataReader objreader = objcmd.ExecuteReader();
objreader.Read();
{
DropDownList1.Items.Add(new ListItem(objreader.GetString(1)));
}